Journal:

Article Title: Members of the 30- to 32-Kilodalton Mycolyl Transferase Family (Ag85) from Culture Filtrate of Mycobacterium avium subsp. paratuberculosis Are Immunodominant Th1-Type Antigens Recognized Early upon Infection in Mice and Cattle

doi: 10.1128/IAI.74.1.202-212.2006

Figure Lengend Snippet: (A) Comparative SDS-PAGE (12%) of culture filtrates from M. avium subsp. paratuberculosis ATCC 19698 (lane 3) and M. tuberculosis H37Rv (lane 2) grown as surface pellicles on synthetic Sauton medium. Proteins were visualized by silver nitrate staining. Molecular weight markers are shown in lane 1. (B) Silver nitrate staining of purified, recombinant E. coli-derived antigen 85A (lane 4) and Ag85B (lane 5) from M. avium subsp. paratuberculosis separated by 12% SDS-PAGE. (C) Western blot analysis of CF from M. avium subsp. paratuberculosis (lane 6) and of recombinant Ag85A (lane 7) and Ag85B (lane 8) from M. avium subsp. paratuberculosis using Ag85-specific MAb TD 17-4. The arrows indicate the position of the 30-kDa molecular weight marker (A and B) and of the native Ag85B protein in M. avium subsp. paratuberculosis CF (C).

Article Snippet: Genomic DNA of M. avium subsp. paratuberculosis ATCC 19698 was prepared as described previously by Tanghe et al. for Mycobacterium ulcerans ( 47 ).

Techniques: SDS Page, Staining, Molecular Weight, Purification, Recombinant, Derivative Assay, Western Blot, Marker

Journal:

Article Title: Members of the 30- to 32-Kilodalton Mycolyl Transferase Family (Ag85) from Culture Filtrate of Mycobacterium avium subsp. paratuberculosis Are Immunodominant Th1-Type Antigens Recognized Early upon Infection in Mice and Cattle

doi: 10.1128/IAI.74.1.202-212.2006

Figure Lengend Snippet: Bovine proliferative responses (mean cpm ± SD) of unstimulated PBMCs (Control) or of PBMCs stimulated with culture filtrate from M. avium subsp. paratuberculosis ATCC 19698 and Ag85 from M. bovis BCG following oral infection with 109 CFU of M. avium subsp. paratuberculosis ATCC 19698 in five animals, as analyzed over a follow-up period of 125 weeks postinfection.

Article Snippet: Genomic DNA of M. avium subsp. paratuberculosis ATCC 19698 was prepared as described previously by Tanghe et al. for Mycobacterium ulcerans ( 47 ).

Techniques: Infection

Journal:

Article Title: Members of the 30- to 32-Kilodalton Mycolyl Transferase Family (Ag85) from Culture Filtrate of Mycobacterium avium subsp. paratuberculosis Are Immunodominant Th1-Type Antigens Recognized Early upon Infection in Mice and Cattle

doi: 10.1128/IAI.74.1.202-212.2006

Figure Lengend Snippet: Ex vivo IFN-γ production in unstimulated cultures (Control) or in cultures stimulated with Ag85 of whole blood following oral infection with 109 CFU of M. avium subsp. paratuberculosis ATCC 19698 in five animals, as analyzed over a follow-up period of 125 weeks postinfection. Data are represented as optical density values (mean ± SD).

Article Snippet: Genomic DNA of M. avium subsp. paratuberculosis ATCC 19698 was prepared as described previously by Tanghe et al. for Mycobacterium ulcerans ( 47 ).

Techniques: Ex Vivo, Infection

Journal:

Article Title: Effect of Three Factors in Cheese Production (pH, Salt, and Heat) on Mycobacterium avium subsp. paratuberculosis Viability

doi:

Figure Lengend Snippet: Inactivation curves for M. avium subsp. paratuberculosis in acetate buffer (50 mM, pH 4) with various salt concentrations (0, 2, 4, and 6% [wt/vol]) at 20°C. (A) Dominic. (B) ATCC 19698.

Article Snippet: Two M. avium subsp. paratuberculosis strains (ATCC 19698 and Dominic) were tested.

Techniques:

Journal:

Article Title: Effect of Three Factors in Cheese Production (pH, Salt, and Heat) on Mycobacterium avium subsp. paratuberculosis Viability

doi:

Figure Lengend Snippet: D values for M. avium subsp. paratuberculosis strains tested at pH 4, 5, and 6 at 20°C

Article Snippet: Two M. avium subsp. paratuberculosis strains (ATCC 19698 and Dominic) were tested.

Techniques:

Journal:

Article Title: Effect of Three Factors in Cheese Production (pH, Salt, and Heat) on Mycobacterium avium subsp. paratuberculosis Viability

doi:

Figure Lengend Snippet: D values for heat-treated M. avium subsp. paratuberculosis strains at pH 5, 2% (vol/vol) NaCl, and 20°C

Article Snippet: Two M. avium subsp. paratuberculosis strains (ATCC 19698 and Dominic) were tested.

Techniques:

Journal:

Article Title: Effect of Three Factors in Cheese Production (pH, Salt, and Heat) on Mycobacterium avium subsp. paratuberculosis Viability

doi:

Figure Lengend Snippet: Inactivation curves for nonheated and heat-treated (at 62°C for 240 s) M. avium subsp. paratuberculosis strain ATCC 19698 in the acetate buffer (pH 5, 2% [wt/vol] NaCl) at 20°C.

Article Snippet: Two M. avium subsp. paratuberculosis strains (ATCC 19698 and Dominic) were tested.

Techniques:

Journal:

Article Title: Effect of Three Factors in Cheese Production (pH, Salt, and Heat) on Mycobacterium avium subsp. paratuberculosis Viability

doi:

Figure Lengend Snippet: Inactivation curves for nonheated and heat-treated (at 62°C for 240 s) M. avium subsp. paratuberculosis strain ATCC 19698 in the soft white cheese at 4°C.

Article Snippet: Two M. avium subsp. paratuberculosis strains (ATCC 19698 and Dominic) were tested.

Techniques: